detection of dna gyrase mutation and multidrug efflux pumps hyperactivity in ciprofloxacin resistant clinical isolates of pseudomonas aeruginosa
نویسندگان
چکیده
target modification and reduced drug accumulation are the main resistance mechanisms against fluoroquinolone antibiotics in pseudomonas aeruginosa. we performed a genotypic characterization of three major mex multidrug efflux pumps (mexab-oprm, mexxy-oprm and mexcd-oprj) in ciprofloxacin resistant clinical isolates of p. aeruginosa, collected from tehran, iran this was followed by sequencing and analyzing the type ii topoisomerases encoding gyra, gyrb, parc , and pare genes. reverse transcription pcr (rt-pcr) and semi-quantitative rt-pcr methods were used to analyse the transcription of efflux pumps. topoisomerase mutation analysis was carried out through pcr amplification and sequencing of the quinolone resistance determining region (qrdr) of the topoisomerases encoding genes. some 11.1% of the strains actively expressed mexcd-oprj and 15.5% hyperexpressed mexxy-oprm efflux pumps. no overexpression was detected for mexab-oprm, whereas 4.4% of strains showed simultaneous expression of mexcd-oprj and mexxy-oprm. in the sequencing results, a single point mutation in the qrdr of gyra was detected in all tested strains, where isoleucine was substituted by threonine at position 83. no mutations were detected in qrdr of gyrb, parc and pare genes. we are the first to report the genotypic analysis of ciprofloxacin mediated efflux pump resistance from iran. these findings emphasize the clinical significance of multidrug efflux pumps and topoisomerases mutations activity in conferring resistance to fluoroquinolone antibiotics, and support the use of genotypic methods for analysis of resistance elements in p. aeruginosa in developing countries such as iran.
منابع مشابه
Detection of DNA Gyrase Mutation and Multidrug Efflux Pumps Hyperactivity in Ciprofloxacin Resistant Clinical Isolates of Pseudomonas aeruginosa
Target modification and reduced drug accumulation are the main resistance mechanisms against fluoroquinolone antibiotics in Pseudomonas aeruginosa. We performed a genotypic characterization of three major Mex multidrug efflux pumps (MexAB-OprM, MexXY-OprM and MexCD-OprJ) in ciprofloxacin resistant clinical isolates of P. aeruginosa, collected from Tehran, Iran this was followed by sequencin...
متن کاملDetection of Multidrug Resistant Efflux Pumps in Clinical Isolates of E.coli in Tertiary Care Hospital
Fluoroquinolones are widely used in both community and hospital settings. E.coli exhibits multiple resistant mechanisms; recent studies showed that multidrug resistant (MDR) efflux mechanism is most common. The effects of MDR inhibitors of the RND efflux pumps, Reserpine 25 μg/ml and MC 207, 110 (Phe-Arg-beta-naphthylamide (PaβN), Sigma) 25 μg/ml, on the MIC of fluoroquinolones for clinical iso...
متن کاملنقش ژن mexZ در مقاومت به سیپروفلوکساسین در جدایه های سودوموناس آئروژینوزا در استان گیلان
Messadi AA, Lamia T, Kamel B, Salima O, Monia M, Saida BR. Association between antibiotic use and changes in susceptibility patterns of Pseudomonas aeruginosa in an intensive care burn unit: a 5-year study, 2000-2004. Burns :J Int Soc Burn Injuries 2008;34(8):1098-102. Sherertz RJ, Sarubbi FA. A three-year study of nosocomial infections associated with Pseudomonas aeruginosa. J Clin Micr...
متن کاملEvaluation of efflux pumps gene expression in resistant Pseudomonas aeruginosa isolates in an Iranian referral hospital
BACKGROUND AND OBJECTIVES Pseudomonas aeruginosa (PA) is one of the most important causes of nosocomial infections and has an intrinsic resistance to many antibiotics. Among all the resistance-nodulation-division (RND) pumps of P. aeruginosa, MexAB-OprM is the first efflux pump found to target multiple classes of antibiotics. This study was aimed to evaluate the expression level of genes expres...
متن کاملEvaluation of the β-Lactamase Disk Test Method in the Detection of Extended-Spectrum-β- Lactamases in Clinical Isolates of Multidrug-Resistant Pseudomonas aeruginosa
Background & Aims: The production of extended-spectrum-β-lactamases (ESBLs) is the main mechanism of resistance to β-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to β-lactam antibiotics caused a decrease in sensitivity of the confirmatory tests for ESBL. The aim of this study was the evaluation of the β-lactamase disk test method in the de...
متن کاملMultidrug-Resistant Virulence Genes in Isolates of Pseudomonas aeruginosa in Iranian Clinical Samples: A Review-Meta-Analysis
Background and purpose: Pseudomonas infections include urinary tract infections (UTIs), respiratory infections, soft tissue infections, dermatitis, bacteremia, gastrointestinal, bone and joint infections, and systemic types of infections, especially in hospitalized patients with severe burns, or patients with immunosuppression such as cancer or AIDS. Considering the importance of the virulence...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of medical microbiology and infectious diseasesجلد ۱، شماره ۱، صفحات ۱-۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023